Protein Labelling with Versatile Phosphorescent Metal Complexes for Live Cell Luminescence Imaging

TU Connell; JL James; AR White; PS Donnelly

Journal article

Protein Labelling with Versatile Phosphorescent Metal Complexes for Live Cell Luminescence Imaging

TU Connell, JL James, AR White, PS Donnelly

Chemistry (Weinheim an der Bergstrasse, Germany) | Published : 2015

DOI: 10.1002/chem.201501630

Abstract

© 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.To take advantage of the luminescent properties of d(6) transition metal complexes to label proteins, versatile bifunctional ligands were prepared. Ligands that contain a 1,2,3-triazole heterocycle were synthesised using Cu(I) catalysed azide-alkyne cycloaddition "click" chemistry and were used to form phosphorescent Ir(III) and Ru(II) complexes. Their emission properties were readily tuned, by changing either the metal ion or the co-ligands. The complexes were tethered to the metalloprotein transferrin using several conjugation strategies. The Ir(III)/Ru(II)-protein conjugates could be visualised in cancer cells using live cell imaging for e..

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University of Melbourne Researchers

Paul Donnelly Author

Related Projects (1)

SITE-SPECIFIC CONJUGATION OF ZIRCONIUM AND COPPER COMPLEXES TO ANTIBODIES FOR APPLICATION IN DIAGNOSTIC IMAGING AND THERAPY

This project will focus on fundamental chemistry that underpins innovations in the synthesis of metal-based imaging and therapeutic agents. ..

Grants

Funding Acknowledgements

The authors thank Associate Professor Trevor A. Smith (University of Melbourne) for expert guidance on luminescence spectroscopy. The Australian Research Council (ARC) is acknowledged for funding. P.S.D. is an ARC Future Fellow.